2024 Top/fop reporter assay

Top/fop reporter assay

Author: jfyp

August undefined, 2024

WebLuciferase reporter assays allow you to investigate promoter activity by measuring light output from luciferase enzyme that is expressed under the control of your promoter of interest. Luciferase genes vary in stability, light output and spectral characteristics, and reporter kits are available with different luciferases for both single- and dual-spectral … Web17. júl 2024 · TOP/FOP-flash reporter assays Cells were seeded in a 96-well plate, and the TOP/FOP-Flash (Genechem) was co-transfected into cells along with LINC01278 siRNA or overexpression plasmid. Luciferase activity was measured using the Promega Dual …

TOP/FOP-FLASH assay in HEK293T cells?

Web11. apr 2024 · We therefore performed a TOP/FOP luciferase reporter assay to monitor β-catenin transcriptional activity in the nucleus of SW620 and Difi cells. The nuclear transcriptional activity of β-catenin was significantly increased by oxaliplatin treatment in both cell lines (Fig. 3I and Supplementary Fig. 2D). Web29. jún 2024 · TOP/FOP-Flash luciferase reporter assay and western blot were used to analysis the activation of Wnt/β-catenin signaling pathway. Si-CASC11-transfected HeLa cells were subcutaneously inoculated into male athymic (nude) mice to investigate the effect of CASC11 on the tumor formation. Results: cupon istyle

Luciferase Assay System Protocol - Promega

WebI performed a dual luciferase assay using pGL3 firefly luc as experimental reporter and pRL-TK renilla luc as internal control (Promega assay kit). I used a manual luminometer to analyze my... Web15. aug 2013 · Using lentiviral wild-type (Top) and mutant (Fop) TCF/LEF reporters, we investigated the status of Wnt signaling in cells derived from several types of human cancer. As a result of this screen, we identified up-regulated TCF/LEF activity in different … WebJournal: PLoS ONE Article Title: The natural compound Jatrophone interferes with Wnt/β-catenin signaling and inhibits proliferation and EMT in human triple-negative breast cancer doi: 10.1371/journal.pone.0189864 Article Snippet: The activity of β-catenin dependent … easy cinnamon rolls allrecipes

β-Catenin/LEF-1 transcription complex is responsible for

β-catenin activation down-regulates cell-cell junction

Web2. apr 2013 · TamR cells exhibited increased Wnt signalling as measured via the TOP/FOP Wnt luciferase reporter assays. Genes associated with both the β-catenin dependent (AXIN2, MYC, CSNK1A1) and independent arms (ROR2, JUN), as well as general Wnt secretion (PORCN) of the Wnt signalling pathway were upregulated in the TamR cells compared to … Web15. feb 2012 · All FOP individuals harbor conserved point mutations in ACVR1 gene that are thought to cause ACVR1 constitutive activation and activate BMP signal pathway. ... Here, we verified that miR-148a directly targeted the 3' UTR of ACVR1 mRNA by reporter gene assays and mutational analysis at the miRNA binding sites, and inhibited ACVR1 both at … cup online ishockeyWebFOP-flash reporter was adopted as the control of TOP flash reporter. g The protein expressions of β-catenin, cyclin D1 and c-myc were measured by western blot in sh-NC or sh-circAGFG1#1 group. h The nuclear translocation of β-catenin in sh-NC or sh-circAGFG1#1 group was assessed by IF assay (scale bar = 20 μm). i Luciferase reporter assay ... cupon irresistible walmart

"Web12. júl 2024 · The Dual-Luciferase Reporter Assay (Promega) was applied according to the manufacturer’s instructions. For the TOP/FOP-Flash assay, TOP/FOP-Flash (Genechem) was co-transfected into cells along with LUCAT1 silence or overexpression vector, miR-5582-5p mimic or inhibitor, and/or the miRNA control. " - Top/fop reporter assay

Top/fop reporter assay

TOPflash (TCF Reporter Plasmid) 21-170 - Merck Millipore

WebFrom what I can understand, TOPflash is a luciferase reporter plasmid that contains two sets of 3 copies of the wild-type TCF binding regions. If the canonical Wnt signaling is activated, beta-catenin will translocate to the nucleus to associate with TCF/LEF … WebPlasmid TOP-GFP from Dr. Ramesh Shivdasani's lab contains the insert 7xTOP-GFP and is published in Cancer Res. 2012 Feb 8. This plasmid is available through Addgene. ... FOP-GFP TOP-GFP.mC Lentiviral-TOP-dGFP-reporter FOP-GFP.mC M50 Super 8x TOPFlash ...

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WebWe offer a full range of luciferase assay products and tools for your research. Reporter options include NanoLuc®, firefly and Renilla luciferases, which have different protein sizes, reporter stabilities and brightness. Detect the luciferase reporter using lytic or live-cell … WebThe LEADING LIGHT ® Wnt Reporter Assay Starter Kit is a cell-based luciferase activity test suitable for 96 well plate format. The system contains an engineered 3T3 mouse fibroblast cell line, which expresses the firefly luciferase reporter gene under the control of Wnt-responsive promoters (TCF/LEF).

Web20. mar 2024 · c–e TOP/FOP flash reporter assay in 293T cells transfected with WT Gfi1b and the indicated mutated forms. Cells were treated with 25 mM LiCl (to activate canonical Wnt signaling in c and e ) for ... Web22. sep 2024 · The luciferase reporter assay is commonly used as a tool to study gene expression at the transcriptional level. It is widely used because it is convenient, relatively inexpensive, and gives quantitative measurements instantaneously. It has broad applications across various fields of cell and molecular biology—wherever you want to …

WebPočet riadkov: 10 · TOPflash (TCF Reporter Plasmid) Transfection grade T-cell factor reporter plasmid containing 2 sets (with the second set in the reverse orientation) of 3 copies of the TCF binding site (wild type) upstream of the Thymidine Kinase minimal … Web2. apr 2024 · 胃癌细胞系MGC-803购自ATCC；SGC-7901细胞购自中国科学院细胞库；抗PRRX2、β-catenin、c-Myc、cyclin D1和GAPDH I抗以及HRP标记的山羊抗兔Ⅱ抗均购自ProteinTech；转染试剂脂质体Lipofectamine 3000(Invitrogen)；胰蛋白酶(索莱宝生物科技有限公司)；MTT细胞活力检测试剂盒(碧云天生物 ...

Web1. mar 2024 · g DAX1-silenced HeLa and SiHa cells were transfected with the TOP/FOP-Flash reporter plasmid, and the reporter activities were detected 48 h after transfection by a luciferase assay.

cupon itevelsaWeb5. dec 2024 · TOP-Flash contained a wild type LEF/TCF-binding site, while FOP-Flash contained a mutant LEF/TCF-binding site. HCT116 cells were transfected with reporter constructs (TOP-Flash or FOP-Flash). cup online fvgWeb( A ) TOP/FOP luciferase reporter assays of 8988T cells treated with vitamin C (4 or 5 mM) for 24 h. ( B , C ) PDAC cells were treated with vitamin C (4 or 5 mM) for 24 h and immunoblotted with the indicated antibodies. Error bars reflect the s.d. of three … cupon hoodie rappiWebHello, I recently started using the TOPFlash assay and I want a positive control to make sure the vectors are working. I read several places that LiCl can be used to activate Wnt signaling. cup onionWebLuciferase assay HEK 293-TOP cells (1 x 104) or Jurkat-TOP/FOP (2 x 104) cells were seeded into 96-well plates and incubated in medium with 10% FBS for one day. The cells were then stimulated... easy cinnamon rolls for kidsWeb26. dec 2024 · topflash is a tcf reporter plasmid containing two sets of three copies of wild-type tcf binding sites driven by the thymidine kinase minimal prompter and upstream of a luciferase reporter gene. fopflash contains mutated tcf binding sites driven by the same … cup on line apsshttp://forpost-audit.ru/ easy cinnamon rolls made with puff pastry