T4 dna ligase qiagen
Web`T4 DNA polymerase `Klenow DNA polymerase `T4 PNK `Water User-Supplied `ChIP enriched, QPCR verified DNA (approx.10 ng in 30 μl water) `QIAquick PCR Purification Kit (QIAGEN, part # 28104) Procedure1. Dilute Klenow DNA polymerase 1:5 with water for a final Klenow concentration of 1U/μl. 2. WebSource of Protein: A recombinant E. coli strain carrying the cloned T4 DNA Ligase gene. Unit Definition: 1 unit is defined as the amount of DNA Ligase required to join 50% of 100 ng of DNA fragments with cohesive termini in 50 µl 1X DNA Ligase Buffer following a 30 minute incubation at 23°C. Molecular weight: 55,292 Daltons. Quality Control ...
T4 dna ligase qiagen
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WebMay 11, 2024 · Conditions amplificationwere followed TOPOTA cloning manual (Invitrogen) onecycle 25cycles eachcycle consisting finalextension productswere analyzed 1%agarose gel after staining PCRproduct containing azoCORF directlycloned pCR2.1-TOPO TA vector (Invitrogen) TOPOclone pET15b(Novagen) were each cleaved sequentialdigestion … Web阿里巴巴为您找到256条供应dna酶试剂产品的详细参数，实时报价，价格行情，优质批发/供应等信息。
WebT4 DNA ligase is an enzyme that fixes broken DNA and seals it – similar to super glue. This particular DNA ligase was isolated from bacteriophage T4. During DNA replication or recombination, a break or a ‘nick’ in the backbone of DNA frequently occurs. Afterwards, the DNA ligase comes in and plays an important role on repairing these ... WebMar 3, 2024 · QIAGEN: Cat#28104: T4 DNA ligase: MGI: Cat#1000004279: PNK buffer: NEB: Cat#B0201S: SPRI beads purification reagent (AMPure XP Reagent) ... and incubating with 50 μL of 1X TA buffer (Teknova, T0380) at 37°C for 1 h. T4 DNA ligase-mediated circularization was performed in 150 μL of 1X TA buffer with extra 1 mM ATP. All …
http://www.enzymatics.com/wp-content/uploads/2014/12/L6030-HC-L-REV-F-T4-DNA-Ligase-PSF-EFF04SEP2014.pdf WebIf you would like to customize a product formulation from the web or if you have a need for a custom reagent that is not available on our website please email us at [email protected] and we would be happy to discuss your needs for custom products. All Products Binding Proteins DNA Modifying Enzymes Kits New Products
WebJan 16, 2014 · T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5′ phosphate and a 3′ hydroxyl groups of duplex DNA or RNA. The enzyme …
WebSep 12, 2016 · Infectious plasmid DNA with both CMV and T7 promoters was constructed by anchored-PCR. PCR was performed using pCMV-T7-F and pCMV-T7-R primers with the pCMV-HH-EV71-HDV backbone. The PCR product was then treated with T4 ligase-T4 polynucleotide kinase-DpnI before transformation into XL10-GOLD ultracompetent cells. … chelsea cantuWebSupplier: Qiagen Beverly Inc L6030HCL DNA Ligase; T4 DNA Ligase (Rapid); Conc. 600,000 U/ml; 240,000U; incl. 10X buffer and 2X rapid ligation buffer Catalog No. 50-305-905 flexbrew moviehttp://www.enzymatics.com/pdf2/L6030-HC-L.pdf flexbrewr trio coffee maker black stainlessWeb1 uL T4 DNA Ligase 4 uL H 2 0 (to bring total volume to 10 uL) Tip: Try different vector to insert ratios to optimize ligation reaction. Tip: Do negative controls: vector only, insert only, no ligase. b. Incubate at 16 o C for 2 hours, or at 4 … flexbrew podsWebIt is not active against single stranded DNA or RNA and blunt ended DNA. Enzyme is isolated from Escherichia coli strain containing plasmid carrying the Thermus thermophilus DNA ligase gene. Tth DNA Ligase is stable and active in optimum ligation temperature range of 45–65°C, which is 7–10°C higher than that of T4 DNA ligase. chelsea cannot sell ticketsWebThe T4 DNA ligase was detected with a linear range of 4.8 × 10-3 to 6.0 U mL-1, achieving a detection limit of 1.6 × 10-3 U mL-1. The outcome indicated that the developed … chelsea cantiehttp://www.lianshimall.com/goods-6957597.html chelsea candi