Rpl22-ha
WebIllustrated plasmid map in PNG format. GenBank File: Plasmid sequence and annotations. Use text editor or plasmid mapping software to view sequence. SnapGene File: Plasmid … WebMestrado em Biologia AplicadaO osteossarcoma ou sarcoma osteogénico (OS) é o tumor maligno primário mais comum de origem óssea que ocorre em crianças e adultos jovens.
Rpl22-ha
Did you know?
WebDec 23, 2024 · In turn, Rpl22-HA protein expression was assessed by Western blotting, revealing high expression in intestinal and brain lysates of used mouse strains, whereas only small amounts were present in the spleen resulting either from Sox10 + astrocyte-like glia cells 25 or ChAT + T cells, 26 respectively. WebSep 28, 2024 · (A) Tie2-Cre; Rpl22HA/HA ( Ribo-Tag) mice, which are designed for expressing HA-tagged ribosomal protein in BECs. (B) Immunohistochemistry for Ai9 (Cre …
WebÐÏ à¡± á ÀF> þÿ ä ã þÿÿÿæ ç è é ê ë ì í î ï ... WebAug 18, 2009 · The RPL22 ha C-Terminal Hemagglutinin Tag Can Be Used To Immunoprecipitate Polysomes. Fig. 2 is a cartoon that outlines the RiboTag methodology. …
WebThe RiboTag mouse has a modified Ribosomal Protein L22 (RPL22), which is one of the 79 core proteins that constitute the ribosome particle as it is assembled in the nucleolus. The final coding exon 4 of the Rpl22 gene is … WebJun 18, 2024 · The Rpl22-HA gene can also be introduced into the animal by stereotaxic injection of an AAV-DIO-Rpl22-HA that is then activated in Cre-expressing cells. Both methods for tagging ribosomes facilitate the immunoprecipitation of ribosome-bound mRNAs and their analysis by qRT-PCR or RNA-Seq. This protocol will discuss the …
WebNotably, Rpl22-HA is expressed under the control of its own promoter. Thus, in contrast to analog systems in which reporter protein expression is proportional to Drd2 promoter …
WebApr 8, 2015 · The percentage of Kiss1 neurons that also express Rpl22-HA is shown in Figure 4, B and D, for each section. Cell counts showed that an average of 27% of Kiss1-positive neurons expressed the HA tag in Pomc-Cre(BAC1):RiboTag mice, whereas in Pomc-Cre(BAC2):RiboTag mice, 59% of the total Kiss1 neurons were Rpl22-HA positive right eye is osWebSingle-cell RNA-sequencing, ribosome-associated mRNA profiling and chromatin analyses, combined with electrophysiology, reveal that FOS activates the transcription of Scg2, a gene that encodes... right eye is not citedWebpZac2.1-GfaABC1D-Rpl22-HA: Ribosomal protein L22 (Mus musculus) Khakh Add to Cart 166079: pNSU299_RPL22A: Promoter of RPL22A (Saccharomyces cerevisiae) Burgess Add to Cart 170317: P-Ef1a-RPL22-3XHA-P2A-EGFP … right eye is blurry but left is okWeb011029 B6N.129- Rpl22 tm1.1Psam /J The RiboTag targeted mutation of the ribosomal protein L22 locus allows Cre-mediated hemagglutinin epitope tagging of ribosomes from … right eye is blurry visionWebMay 31, 2024 · After collecting the PBL, active mRNAs bound to the Rpl22 HA were isolated with anti-HA antibody-mediated ribosome pulldown and sequenced. (B) Immunohistochemistry of the PBL region of the Oprm1 Cre:Rpl22 HA mice with anti-GFP and anti-HA antibodies showed that Cre:GFP and Rpl22 HA were coexpressed in PBL Oprm1 … right eye is twitching and blurryWebSequences pZac2.1-GfaABC1D-Rpl22-HA Sequences (2) Addgene Sequences: Full (1) Depositing Scientist Sequences: Full (1) Full Sequences from Addgene (1) Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence). … right eye injectionWebApr 6, 2024 · 在整体动物模型研究中，通过RiboTag小鼠与表达细胞类型特异性 Cre 重组酶的小鼠杂交，可获得细胞类型特异的HA标记核糖体亚单位蛋白Rpl22的基因修饰工具鼠，解剖分离特定组织进行TRAP-seq，可获得特定组织、特定细胞类型的翻译组数据。 right eye issues