Splet06. sep. 1993 · Hence, bacteria carrying pBT7 without an insert form blue colonies on an indicator plate, while bacteria carrying recombinant clones with an insert appear as white … Splet01. jun. 2024 · It confirmed that clone C-2a of E. coli contained plasmid pBT7-N-His (4.001 bp) inserted by recombinant F protein of NDV gene (642 bp). The visualisation of …
SciELO - Brasil - A high-copy T7 Escherichia coli expression vector …
SpletTransformation recombinant plasmid pBT7 -N His F into E. coli BL21(DE3) The recombinant plasmids were transformed into E. coli BL21(DE3) competent cells by heat shock method. The cells were inoculated on LB medium with ampicillin (50 μg.mL-1), then incubated at 37°C for 18 h. The colonies SpletThe DNAs were inserted into vector pBT7-C-His (Bioneer) to construct recombinant expression vectors, which were named pBT7-C-His-CJ_Pfe, pBT7-C-His-CJ_Twi, pBT7-C … henny in a paper bag
PENGARUH KONSENTRASI IPTG DAN KEPADATAN SEL …
Splet[그림] pBT7-C-His vector 클로닝 [그림] Comassie blue stain을 통한 ZIKV NS1 단백질의 발현 [그림] Western blotting을 통한 ZIKV NS1 단백질의 발현 [그림] ZIKV NS1 단백질의 정제 … SpletThe DNAs were inserted into vector pBT7-C-His (Bioneer) to construct recombinant expression vectors, which were named pBT7-C-His-CJ_Pfe, pBT7-C-His-CJ_Twi, pBT7-C … SpletCROSS REFERENCE TO RELATED APPLICATIONS. The present application is a continuation-in-part of International Application No. PCT/KR2024/003769, filed Mar. 30, 2024, which … henny hustle