Nettet19. feb. 2024 · In situ whole mount HCR V3 was performed as previously described 21 using reagents from Molecular Instruments. In brief, each condition (up to 100 gastruloids) ... NettetIn situ HCR v3.0: multiplexed quantitative mRNA imaging in thick autofluorescent samples with automatic background suppression throughout the protocol ( Choi et al., 2024 ). Multiplexing timeline: the same two-stage enzyme-free protocol is used independent of the number of target mRNAs ( Choi et al., 2024 ).
Multiplexed Quantitative In Situ Hybridization with Subcellular or ...
NettetHybridization Chain Reaction Hcr V3 0 Probe Sets, supplied by Molecular Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. … NettetHCR v1.0 1st generation in situ HCR technology (v1.0) using RNA HCR probes and RNA HCR amplifiers: multiplexed mRNA imaging in whole-mount vertebrate embryos with … iphone obs camera
Molecular Technologies
NettetHCR™ RNA-FISH Technology. Multiplexed, quantitative, high-resolution RNA imaging. HCR™ probe sets, amplifiers, and buffers enable multiplexed, quantitative, high … Here, we describe an optimized protocol of whole mount multiplexed RNA in situ hybridization chain reaction version 3.0 (HCR v3.0) in combination with immunohistochemistry (IHC), followed by fructose-glycerol clearing and light sheet fluorescence microscopy (LSFM) imaging on Octopus vulgaris embryos. Se mer The recently increased availability of genomic information has spurred molecular research on several cephalopod species, including … Se mer Our aim was to report an optimized protocol for whole mount HCR v3.0 and its compatibility with IHC as well as a water-based tissue clearing method on octopus embryos for understanding neural anatomy and … Se mer In this study, we report for the first time on optimization of whole mount RNA multiplexed in situ hybridization chain reaction (HCR) combined with immunohistochemistry, … Se mer NettetAssociate the HCR file extension with the correct application. On , right-click on any HCR file and then click "Open with" > "Choose another app". Now select another program … iphone obs つなぎ方