WebMethoCult H4100 or H4236 are used for cell lines and primary CD34+ cells respectively. Colonies are detected after 10 days of culture by adding 1 mg/mL of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent and are scored by Image J quantification software. Animal Research: Animal Models: mouse xenograft model of … Web28 nov. 2024 · MethoCult H4100 (Cat#04100, STEMCELL Technologies) 【Instruments and Equipment】 Cell counter plate (disposable) (Cat# WC2-100S, Waken BTech Co., Ltd) Cellbanker 1 plus, cryopreservation solution for culture cells (Cat#CB021, ZENOAQ) CryoTube vials (Cat#377267, Thermo Fisher Scientific)
4100 - Description : MEDIA METHOCULT H4100 FOR HUMANCELL …
WebMethoCult™ H4100 is suitable for the growth and enumeration of hematopoietic progenitor cells in colony-forming unit (CFU) assays of human bone marrow, mobilized peripheral … WebTo establish a protocol that is simple, reproducible and compatible with hematopoietic cell culture, two commercial methylcelluloses (MethoCult H4100 and SF H4236) developed … strictly dancers 2020
MethoCult™ H4100/基础甲基纤维素培养基
Web4100 - Description : MEDIA METHOCULT H4100 FOR HUMANCELL 40ML - MEDIA METHOCULT H4100 FOR HUMANCELL 40ML - Each. Hi! Sign in or register. Follow us … Webdium (Methocult H4100, StemCell Technologies) diluted in α-MEM culture medium containing 10 % FBS to a final concen-tration of 1 % and mixed by pip etting. Then, the … Web1 aug. 2024 · For HL60 human cells, H4100 Methocult® medium (Stem Cell Technologies, USA) was used. Cells were cultured in the presence of Cafestol (40 μM and 80 μM) and … strictly dancers female