2024 Fatb1

Fatb1

Author: mbag

August undefined, 2024

Tīmeklis2024. gada 1. maijs · On the basis of this modeling, we transplanted putative ACP interaction sequences from CpFatB1 into CpFatB2 and created a chimeric … TīmeklisNational Center for Biotechnology Information

Characterization of a Palmitoyl-Acyl Carrier Protein Thioesterase ...

Tīmeklis2024. gada 1. maijs · The thioesterase (TE) FatB1 from C. palustris (CpTE) contains two domains, an N-terminal Acyl-thio_N domain (pfam12590) and a C-terminal Acyl-ACP-TE domain (pfam01643) (Fig. 1 a).The C-terminal domain is highly conserved among different classes of TEs, and it is responsible for substrate specificity of the enzyme … TīmeklisGene Model: FATB (OBSOLETE) [ Help] Encodes an acyl-acyl carrier protein thioesterase. Hydrolyzes primarily saturated acyl-ACPs with chain lengths that vary … baseband 4g vs 5g rate

Functional expression analysis of an acyl-ACP thioesterase FatB1 …

Tīmeklis1995. gada 7. nov. · By site-directed mutagenesis, we have replaced several amino acids in Uc FatB1 by using the Cc FatB1 sequence as a guide. A double mutant, which changes Met-197 to an Arg and Arg-199 to a His (M197R/R199H), turns Uc FatB1 into a 12:0/14:0 TE with equal preference for both substrates. Another mutation, T231K, by … TīmeklisTo better understand the role of the FatB class of acyl-acyl carrier protein thioesterase, a 1.2 kb cDNA fragment of AhFatB1 was amplified using RT-PCR method from the … TīmeklisAnalysis of AhFatB1 using CLUXTALX, sequence alignment showed that the amino acid residues had the highest identity with GmFatB (71%), and only 50% identity with … baseband chipset mav 30 maida

Accumulation of Palmitate in Arabidopsis Mediated by …

Modification of the substrate specificity of an acyl-acyl carrier ...

Tīmeklis1995. gada 7. nov. · This TE, Cc FatB1, mainly hydrolyzes 14:0-ACP as shown by E. coli expression. We have investigated the roles of the N- and C-terminal regions in determining substrate specificity by constructing two chimeric enzymes, in which the N-terminal portion of one protein is fused to the C-terminal portion of the other. Our … Tīmeklis2000. gada 1. jūl. · When a FatB1 gene from Cuphea wrightii was over-expressed in Arabidopsis, the content of lauric (C12:0), myristic and palmitic acids were increased, … baseband and bandpassTīmeklis2024. gada 1. jūl. · The objective of this study was to implement direct sunlight-driven conversion of CO 2 into a naturally excreted ready-to-use fuel. We engineered four different synthetic metabolic modules for biosynthesis of short-to medium-chain length hydrocarbons in the model cyanobacterium Synechocystis sp. PCC 6803. In module … baseband attacks

"Tīmeklis2024. gada 9. apr. · The final constructs were named fatb1-Cas9-1 and fatb1-Cas9-2. In addition, we used the Gm FATB1a endogenous promoter to overexpress Gm FATB1 . A fragment containing a 1725-bp promoter of GmFATB1a (ATG upstream of the gene), a TATA region, an Omega sequence, a Flag tag, and a 2999-bp coding region … " - Fatb1

Fatb1

Thioesterase overexpression in Nicotiana benthamiana leaf

Tīmeklis2016. gada 1. maijs · Palmitic acid is a saturated fat linked to increased cholesterol levels, and reducing levels of saturated fats in soybean oil is a target for soybean … TīmeklisDisclosed herein are obligate heterotrophic microalgae cells containing an exogenous gene. In some embodiments the gene is a sucrose utilization gene, and further disclosed are me

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Tīmeklis2024. gada 1. okt. · Furthermore, GA may act through FaTB1 expressed in axillary buds, repressing bud outgrowth and tiller development. The mechanisms of GA-regulation of tiller bud outgrowth through crosstalk with CK and signaling of FaTB1 expression deserve further investigation. Author contributions. LZ and BH planned and designed … Tīmeklis2011. gada 11. apr. · In SD232, f1 and f2 are the flanking regions for deletion of sll1951; is an improved promoter from P psbA2; Uc fatB1 is a TE gene from U. californica; …

TīmeklisFATB1. ORF names. T27G7.19. Ordered locus names. At1g08510. Organism names. Organism. Arabidopsis thaliana (Mouse-ear cress) Taxonomic identifier. 3702 NCBI. …

Tīmeklis2024. gada 1. maijs · The thioesterase (TE) FatB1 from C. palustris (CpTE) contains two domains, an N-terminal Acyl-thio_N domain (pfam12590) and a C-terminal Acyl-ACP … Tīmeklis2024. gada 27. maijs · The palmitoyl-acyl-carrier protein (ACP) thioesterase enzyme (FatB1) transports palmitic acid from the chloroplast into the cytoplasm where it is rapidly incorporated into triacylglycerides (Liu et al. 2009, Yoder et al. 1999). The palmitic acid melts at 63 °C and stearic acid melts at 70 °C (Linder 2000). However, …

Tīmeklis2016. gada 26. dec. · Arabidopsis FATB1 overexpression has been reported to increase the accumulation of palmitate in the Arabidopsis seeds . These and other results indicate that acyl-ACP thioesterases can be key enzymes to produce specialty bioengineered oils not only in seed but also in leaf tissue as well, provided acyl-ACP thioesterases …

Tīmeklis2009. gada 1. okt. · Here, we report the cloning and characterization of a cDNA isolated from J. curcas, designated JcFATB1, which encodes a putative acyl-ACP TE (likely … svu s4e17TīmeklisThe genetic modification results in RNA suppression of FATB1-A and FAD2-1A transcripts which leads to a change in the fatty acid profile in the soybean seed. MON 87705 has an improved fatty acid profile similar to that of canola oil and olive oil, consisting of increased monounsaturated and reduced polyunsaturated and … baseband cpu iphone 7TīmeklisUniProt website fallback message If you are not seeing anything on this page, it might be for multiple reasons: You might have JavaScript disabled: make sure to ... svu s4 e9Tīmeklis2013. gada 8. febr. · The modified fatty acid profile in MON 87705 soybean oil is achieved through the use of endogenous soybean (Glycine max L.) gene segments configured to suppress FATB and FAD2 gene expression. MON 87705 contains FATB1-A and FAD2-1A gene segments under the control of a seed promoter, limiting oil … svu s4e2Tīmeklis2000. gada 1. marts · The best-studied example to date is the laurate-producing rape (Calgene™), in which a California bay tree (Umbellularia californica) medium-chain fatty acid thioesterase (FatB1) was expressed using the seed-specific Napin promoter. In some of these Napin-FatB1 lines, 12:0 accumulated to >55 mol% of the seed … basebandedTīmeklisFunction: decreases the transport of saturated fatty acids out of the plastid, thereby increasing their availability to desaturation to 18:1 oleic acid; reduces the levels of … svu s4 e5TīmeklisDisruption of the FATB gene in Arabidopsis results in a two-thirds reduction in saturated fatty acids, largely palmitate, in the leaf extra-plastidic phospholipids and a reduction … baseband de iphone