By4742 yeast
Web摘要 以酿酒酵母Saccharomyces cerevisiae BY4742为宿主茵,利用DNA组装(DNA assemble)技术,向宿主茵导入了β-胡萝卜素合成途径,表达了源自Xanthophyllomyces dend... 展开更多 By the DNA assembly method, the β-carotene biosynthesis pathway was induced into the host strain, Saccharo- myces cerevisiae BY4742, which successfully expressed … WebJul 28, 2024 · The MATa haploid strain of S. cerevisiae BY4742 (Thermo Fisher Scientific, Waltham, MA, USA) was used as a host for promoter engineering and combinatorial pathway optimization. BY4742 and its derivative strains were routinely cultured in standard Yeast Peptone Dextrose medium (YPD; 1% w/v yeast extract, 2% w/v peptone, and 2% …
By4742 yeast
Did you know?
WebQuestion: Yeast strains, Wild type BY4742 (A) and its MATa knockout mutant (B) (deficient in DNA repair) were plated at different concentrations and were subjected to UV irradiation (except the AO and BO plates) and were incubated at 30°C to observe the role of repair in preventing UV damage to DNA. You are going to compare the survival curves for the … WebOct 6, 2014 · BY4742 (Y10000) yeast wild type strains Reference: Brachmann CB, Davies A, Cost GJ, Caputo E, Li J, Hieter P and Boeke JD Designer deletion strains derived …
WebAug 1, 2024 · The creation of a single-chromosome yeast from S. cerevisiae BY4742 haploid cells required 15 rounds of chromosome end-to-end fusions, with deletion of 15 centromeres and 30 telomeres (Fig. 1a,... WebNov 1, 2024 · The yeast cells that lost the plasmids were grown on a solid YPD medium, yielding strain BY4742A. Strain BY4742 was cultivated with strain BY4741 in 1 mL of …
WebMar 16, 2024 · Yeast strains are in the BY4742 background (MATα his3∆1 leu2∆0 lys2∆0 ura3∆0). The HAT and HDAC deletions in this paper were from MATalpha Library, which generated by a PCR-based gene ... WebBY4742 is part of a set of deletion strains derived from S288C in which commonly used selectable marker genes were deleted by design in order to minimize or eliminate … GenBank GCF_000146045.2 Assembly Size 12157105 Scaffolds 17 Longest …
WebAug 28, 2024 · The results showed that all four driven spores were obviously more thermotolerant than the BY4742 strain was, demonstrating the use of chromosome drive to transmit complex genetic traits in yeast ...
WebSep 21, 2016 · YXM10: strain with cytoplasm engineering; BY4742-M-04, strain with mitochondria engineering; BY4742-MC-01, haploid strain with a mixed cytosolic-mitochondrial strategy; BY4742-C-05, haploid... google play verify cardWebthe BY4742 strains, His+ frequencies peaked after two or three subcultures and then noticeably declined (Fig. 1C, left panel). For the GRF167 strains, the highest His+ fre-quencies were obtained after four to five subcultures, but they then decreased only modestly (Fig. 1C, right panel). The median peak His+ frequencies for the BY4742-de- google play verify identityWebNov 8, 2024 · Similar to our procedure with wild-type Pma1, the Pma1- C22 was first cloned in the yeast strain BY4742 using a PCR-based genomic epitope-tagging method. However, this strain only yielded a small ... chicken burrito bowls budget bytesWebJul 28, 2024 · The MATa haploid strain of S. cerevisiae BY4742 (Thermo Fisher Scientific, Waltham, MA, USA) was used as a host for promoter engineering and combinatorial … chicken burrito casserole kay nutritionWebAug 9, 2010 · S. cerevisiae strain BY4742 cells (MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0) and the respective knockouts in the CTT1 and CTA1 genes (European Saccharomyces cerevisiae Archive for Functional Analysis, EUROSCARF) were used for all experiments. Cell stocks were maintained in YEPD agar medium containing 0.5% yeast extract, 1% … google play version codeWebBY4741 is part of a set of deletion strains derived from S288C in which commonly used selectable marker genes were deleted by design in order to minimize or eliminate … google play version historyWebThe yeast strain BY4742 was cultured at 28 °C in YPD medium (2% glucose, 2% polypeptone, and 1% yeast extract). Yeast transformants were selected on solid SD … google play version