Bmal1 chip-seq
WebAug 20, 2024 · A secondary approach to verify BMAL1 targets identified with ChIP-seq, was to query for overlap of targets with genes that exhibited circadian rhythms of expression in mammary tissue or identified as BMAL1 targets in hepatic tissue. The set of genes in S2 and S3 Tables were used for this analysis. WebDec 13, 2024 · To test whether hepatic BMAL1 also plays a role in the thermogenic response to low-nutrient conditions, we measured body temperature in mice harbouring a floxed allele of Bmal1 before and after...
Bmal1 chip-seq
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WebFeb 23, 2024 · A line of mammary epithelial cells with the BMAL1 86gene knocked out (BMAL1-KO) using CRISPR-CAS was created to verify ChIP-seq findings, 87and study … WebSep 10, 2015 · The published ChIP-seq data also demonstrated the specificity of Bmal1 binding on regulatory regions of predicted target genes, such as NADH dehydrogenase (ubiquinone) 1 alpha subcomplex …
Previously we discovered an extensive genome-wide colocalization of HNF4A and BMAL1::CLOCK in the mouse liver24. While physical interactions and genome-wide co-occupancy between the diurnal regulatory machinery and tissue-specific transcription factors have been reported25,26,27, to our … See more The cooperative loading of transcription factors may involve two mechanisms: (1) a simultaneous loading mediated by protein–protein interactions; (2) a sequential loading … See more The chromatin remodeling activity and rhythmic recruitment24 of HNF4A prompted us to test whether the hepatic chromatin landscape is dynamically shaped throughout … See more We previously showed that Hnf4a knockdown caused varying degrees of circadian rhythm disruption in cell cultures including period shortening and complete arrhythmicity24. Consistently, tissue explants of HKO liver … See more To evaluate how HNF4A activity is supervised by the circadian clock, we first induced chronic circadian disruption in mice by performing a jet lag protocol for 4 weeks. At the end of the treatment, remarkably, night … See more WebTo analyze the effect of PER2 on CLOCK–BMAL1-controlled gene expression, we first defined CLOCK–BMAL1-controlled genes as genes with transcription start sites within ±5 kb from BMAL1 binding sites. …
WebAug 20, 2024 · Deletion of Bmal1 has previously been demonstrated to lead to increased Hif1a mRNA in macrophages, and ChIP-Seq revealed BMAL1 binding to the regulatory regions of the Hif1α promoter . It is possible that we observe increased HIF-1α with deletion of Bmal1 through a lack of BMAL1-mediated suppression of Hif1α mRNA transcription, … WebApr 17, 2024 · Current models of time-specific transcription in the liver involve the core clock transcription factors (TFs) CLOCK:BMAL1 that activate transcription maximally at ZT6 [ 15 – 17] as well as the nuclear receptors RORs and REV-ERBs, whose targets are maximally transcribed around ZT20 [ 18, 19 ].
Webimmunoprecipitation at the genome wide level (ChIP-Seq) in the mouse liver, kidney, and heart. Our data revealed that the majority of BMAL1 DNA binding is tissue-specific, and …
WebDysregulation of circadian rhythms associates with cardiovascular disorders. It is known that deletion of the core circadian gene Bmal1 in mice causes dilated cardiomyopathy. … guardians of the galaxy tank topguardians of the galaxy tape playerWebChIP-Seq: Library source: genomic: Library selection: ChIP: Instrument model: Illumina NovaSeq 6000 : Description: Eluted ChIP DNA from Female mouse at timepoint ZT12 of Bmal1 under the Control protocol. Data processing: ChIP-seq was processed via the nf-core ChIP-seq pipeline v1.2.2 using the narrow peaks method. bouncers bookWebNational Center for Biotechnology Information guardians of the galaxy team upWebChIP-Seq: Library source: genomic: Library selection: ChIP: Instrument model: Illumina NovaSeq 6000 : Description: Eluted ChIP DNA from Female mouse at timepoint ZT0 of Bmal1 under the Misalignment protocol. Data processing: ChIP-seq was processed via the nf-core ChIP-seq pipeline v1.2.2 using the narrow peaks method. guardians of the galaxy teil 1WebBy using an RNA- and ChIP-seq genome-wide approaches we identified that BMAL1 binds the promoters of CCNA1 and CDK1 genes and controls their expression in EC. Conclusions: Our findings show that EC display a robust circadian clock and that BMAL1 regulates EC physiology in both developmental and pathological contexts. Genetic alteration of BMAL1 ... guardians of the galaxy teamWebJun 26, 2024 · Using cell fractionation and mass spectrometry we reveal that BMAL1 associates with NOP58, a core component of the box C/D small nucleolar … guardians of the galaxy tee shirt