WebMar 29, 2024 · Clostridium difficile is one of the main etiological agents causing antibiotic-associated diarrhea. This study investigated the genetic diversity of 70 toxigenic C. difficile isolates from two Korean hospitals by employing toxinotyping, ribotyping, multilocus sequence typing (MLST), and pulsed-field gel electrophoresis (PFGE). Toxin gene … WebApr 14, 2024 · To identify viruses in symptomatic Cz, total RNA was extracted from leaves of a Cz sample using the Plant RNA Prep Kit (Biocubesystem, Korea) and tested by RT …
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WebDec 3, 2024 · Real-time PCR was performed using the SYBR Green Master Kit (BioFACT, Korea), which emits fluorescence to cDNA that can then be detected. Each sample was assayed in triplicate. The Bio-Rad CFX connects Real-time system was used for analysis with the following program: initial denaturation at 95°C for 15min, followed by 40cycles of … WebJul 1, 2024 · Total RNA was extracted from each retina using BioFACT™ Total RNA Prep Kit (Biofact, Korea) according to the manufacturer's instructions. RNA quantity and quality was evaluated with a NanoDrop 2000 Spectrophotometer (Thermo Scientific) and gel electrophoresis. 1 μg of total RNA was reverse transcribed using BioFact™ 5× RT Pre … magda scholl
2024 103:200-213 https://doi.org/10.1094/PDIS-05-18-0857 …
WebJun 9, 2024 · The real-time polymerase chain reaction was performed using BioFact™ 2X Real-Time PCR Master mix kit (BIOFACT) and the StepOne Plus™ quantitative Real time PCR detection System (Applied Biosystems). ... staining with EcoDye™ DNA solution (BIOFACT, Korea), and Finally, under UV light the gel image was captured in E-Box gel … WebBIOFACT Co., Ltd. CEO : Moon Sung Eun V.A.T No : 314-86-36959 ADDRESS : 2/3F,240 EXPO-ro, Yuseong-gu, Daejeon,34124 Republic Korea DAEJEON OFFICE : +82-70 … WebIn archaeology, a biofact (or ecofact) is any organic material including flora or fauna material found at an archaeological site that has not been technologically altered by … magda scott